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Automated single plasma B-cell secretion screening and recovery

Accelerating antibody discovery: Parallel analysis and isolation of thousands of single plasma B cells within one day

The identification and selection of optimal clones that secrete high levels of the desired antibody is one of the critical and time consuming steps in the antibody discovery process.

Our new method for the analysis and isolation of single B-cells uses the CellCelector™ together with the unique Nanowell technology and combines selective cell screening, imaging, sensitive single cell assays as well as cell isolation into a single platform. Single cell analysis allows also the detection of rare antibodies with unique properties which are hard to find under conventionally screening methods. Individual cells will be immediately tested in several assays instead of culturing for weeks to reach a minimum number of cells for assay

CellCelector 1-day B cell screening and isolation workflow

Step 1: Easy cell seeding

The single cell suspension is dispensed manually into the wells of the Nanowell array.

Step 2: Single cell screening

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Step 3: Running the secretion assay

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Step 4: Cell transfer

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Step 4: Generation of the report

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Advantages of the CellCelector™ single plasma B cell workflow

Automation

  • Complete process from cell loading to export of hits with full documentation of the whole screening and cell recovery process


Precision

  • Exact identification of single cells in subnanoliter volume


Throughput

  • Screening of thousand of single B-cells in parallel thanks CellCelector™ Nanowell based approaches

Efficiency

  • Screening process shortened from several months to one day
  • Increased success rate for discovering rare antibodies

Robustness

  • No fusion step required
  • No B-cell loss

Sensitivity

  • Extremely gentle cell transfer guarantees healthy and vital B-cells after screening and sorting for use in downstream applications

Flexibility

  • CellCelector™ platform is capable to work with versatile secretion assays tailored for specific projects
  • In depth characterization: affinity, specificity and functionality



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