Publikationen
Antikörperentwicklung / Nanowell-basiertes B-Zell und T-Zell Screening und Isolierung
- Matochko W.L. et al. CellCelector™ as a platform in isolating primary B cells for antibody discovery Antibody Therapeutics, 5 (1), 11-17 (2022)
- Nelep C. Fast Antibody Discovery Workflows on the CellCelector™ Flex Platform Poster PEGS Europe (2021)
- Bandey I.N. et al. Designed improvement to T-cell immunotherapy by multidimensional single cell profiling Journal for ImmunoTherapy of Cancer; 9:e001877 (2021)
- Pedreño-Lopez, N. et al. An Automated Fluorescence-Based Method to Isolate Bone Marrow-Derived Plasma Cells from Rhesus Macaques Using SIVmac239 SOSIP.664. Methods and Clinical Development 18 (11), 781-790 (2020)
- Sendra, V.G. et al. Detection and isolation of auto-reactive human antibodies from primary B cells Methods 64(2): 153-9 (2013)
- Varadarajan, N. et al. High-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis J. Clin. Invest. 121(11): 4322-31 (2011)
- Choi, J.H. et al. Development and optimization of a process for automated recovery of single cells identified by microengraving Biotechnol. Prog. 26(3): 888-95 (2010)
Zelllinienentwicklung inkl. Nanowell-basierter Einzelzellklonierung
- Tejwani, V. et al. High-throughput and automation advances for accelerating single-cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production (Review). Biotechnol Progress. 2021;e3208. (2021)
- Broussau, S., Nelep, C. & Gilbert, R. Improved Packaging Cell Line Development for Lentivirus Production by CellCelector™ Nanowell-based Single Cell Cloning Technology. Poster Festival of Biologics (2019)
- Winkler, K. et al. Evaluation of the HT-NIC Technology to Generate Monoclonal Producer Cell Lines. Poster ESACT (2019)
- Thiele, M. et al. Generation of Highly Productive Chinese Hamster Ovary Cells by Application of Metabolic Pool Selection During Cell Line Development. Current Biotechnology, 7(5), 2018, 355-367 (2018)
Isolierung adhärenter Zellkolonien (Stammzellen, Tumorzellen, HEK293, etc.)
- Nießing, B. et al. Techno-Economic Analysis of Automated iPSC Production Processes , 9, 240 (2021)
- Maslova, E.A. et al. Functional Evaluation of a Rare Variant c.516G>C (p.Trp172Cys) in the GJB2 (Connexin 26) Gene Associated with Nonsyndromic Hearing Loss Biomolecules, 11, 61. (2021)
- Elanzew, A., et al. The StemCellFactory: A Modular System Integration for Automated Generation and Expansion of Human Induced Pluripotent Stem Cells. Front. Bioeng. Biotechnol.,(2020)
- Belair, D.G. et al. Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4 Sci Rep 10, 2864 (2020)
- Segerman, A. et al. Clonal Variation in Drug and Radiation Response among Glioma-Initiating Cells Is Linked to Proneural-Mesenchymal Transition Cell Reports 17: 2994-3009 (2016)
- Cohen, I.S. et al. DNA lesion identity drives choice of damage tolerance pathway in murine cell chromosomes Nucl. Acids Res. 43(3): 1637-45 (2015)
- Shipony, Z. et al. Dynamic and static maintenance of epigenetic memory in pluripotent and somatic cells Nature 513(7516): 115-9 (2014)
- Marx, U. et al. Automatic Production of Induced Pluripotent Stem Cells Procedia CIRP 5: 2-6 (2013)
- Haupt, S. et al. Automated selection and harvesting of pluripotent stem cell colonies Biotechnol. Appl. Biochem. 59(2): 77-87 (2012)
- Zoldan, K. et al. Automated harvest of induced pluripotent stem cell colonies and colony fractions using the cell separation robot CellCelectorTM nature methods application notes (2010)
- Zoldan, K. et al. Automated isolation of semi-adherent macrophage-like cells from a fibroblast-contaminated culture using the cell separation robot CellcelectorTM nature methods application notes (2010)
- Haupt, S. et al. Automated selection and harvesting of pluripotent stem cell colonies using the CellCelector Nature Methods (2009)
- Schneider, A. et al. "The good into the pot, the bad into the crop!" - a new technology to free stem cells from feeder cells PLoS One 3(11): e3788 (2008)
- Peterbauer, T. et al. Simple and versatile methods for fabrication of arrays of live mammalian cells Lab on a Chip 6(7): 857-63 (2006)
- Planes, E. et al. Life Cell Imaging for Quality Control of Pluripotent Stem Cell Culture Poster ISSCR 2013 Boston
Picken von Klonen aus halbfesten Medien (CHO, Hybridoma, etc.)
- Vaniotis, G. et al. Enhanced anti-metastatic bioactivity of an IGF-TRAP re-engineered to improve physicochemical properties Scientific Reports 8: 17361 (2018)
- Zoldan, K. et al. Automated clonal selection of high-producing hybridoma colonies from methylcellulose-based, semi-solid medium using the cell separation robot CellCelectorTM nature methods application notes (2010)
- Caron A.W. et al. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins (CHO) BMC Biotechnol. 9: 42 (2009)
- Julien, A. et al. Antigen specific antibody-producing clones selected by isotypes Biosystems International Application Note
- Gilbert, R. Fluorescent labeling in Semi-Solid Medium to Identify High Expressing Clones for Recombinant Protein Production (CHO) CHI's Bioprocessing Summit Presentation, Boston 2011
Sphäroidisolierung und Manipulation
- Nilsson Hall G. et al. Cartilaginous spheroid-assembly design considerations for endochondral ossification: towards robotic-driven biomanufacturing Biofabrication 2021 Sep 21;13(4)
Einzelzell CRISPR Gen editing - Einzelzellklonierung
- Chang A. Y. et al. Modulation of SF3B1 in the pre-mRNA spliceosome induces a RIG-I-dependent type I IFN response Journal of Biological Chemistry, 297 (5), , 101277 (2021)
Isolierung seltener Einzelzellen: zirkulierende Tumorzellen (CTCs)
- Acheampong, E, et al. Powering single-cell genomics to unravel circulating tumour cell subpopulations in non-small cell lung cancer patients. J Cancer Res Clin Oncol (2022)
- Diamantopoulou, Z., et al. The metastatic spread of breast cancer accelerates during sleep. Nature 607, 156–162 (2022)
- Yang, L., et al. Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions. Cytometry 2022, 1–11. (2022)
- Ladurner M. et al. Validation of Cell-Free RNA and Circulating Tumor Cells for Molecular Marker Analysis in Metastatic Prostate Cancer Biomedicines 2021 Aug 12; 9(8):1004
- Gjyrezi, A., et al. Androgen receptor variant shows heterogeneous expression in prostate cancer according to differentiation stage Commun Biol 4, 785 (2021)
- Donato C. et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Reports, 32, Issue 10, 2020, 108105
- Franken A., et al. Detection of ESR1 Mutations in Single Circulating Tumor Cells on Estrogen Deprivation Therapy but Not in Primary Tumors from Metastatic Luminal Breast Cancer Patients The Journal of Molecular Diagnostics, 22 (1), , 111-121 (2020).
- Lynch, D., et al. Isolation of Circulating Tumor Cells from Glioblastoma Patients by Direct Immunomagnetic Targeting [CTC imaging and enumerating], Appl. Sci., 10(9), 3338, (2020)
- Ding P.N. et al. The predictive and prognostic significance of liquid biopsy in advanced epidermal growth factor receptor-mutated non-small cell lung cancer: A prospective study Lung Cancer 134:42-45 (2019)
- Sprouse M.L. et al. PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling Int. J. Mol. Sci. 20(8):1916, (2019)
- Bang-Christensenet S.R. et al. Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein Cells 8(9):998, (2019)
- Reinhardt, F. et al. Diagnostic Leukapheresis Enables Reliable Transcriptomic Profiling of Single Circulating Tumor Cells to Characterize Inter-Cellular Heterogeneity in Terms of Endocrine Resistance Cancers (Basel), 11(7), (2019)
- Szczerba, B. et al. Neutrophils escort circulating tumour cells to enable cell cycle progression Nature 566: 553-557 (2019)
- Donato C. et al. Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment J. Vis. Exp. (147), e59677 (2019)
- Gkountela, S. et al. Circulating Tumor Cell Clustering Shapes DNA Methylation to Enable Metastasis Seeding Cell 176 (1-2): 98-112.e14 (2019)
- Cabezas-Camarero, S. et al. RAS analysis of circulating tumor cells from advanced colorectal cancer using BEAMing technology Journal of Clinical Oncology 37 (15_suppl) (2019)
- Kim, D.U. et al. Comprehensive characterization of single circulating rare cells through automated picking and high-throughput mRNA profiling in patients with pancreatic cancer Journal of Clinical Oncology, 37 (4_suppl) (2019)
- Nelep, C. et al. Automated rare single cell picking with the ALS CellCelector Cytometry Part A, cyto.a.23568. [Epub ahead of print] (2018)
- Krol, I. et al. Detection of circulating tumour cell clusters in human glioblastoma British Journal of Cancer 119:487-491 (2018)
- Agerbæk, M.Ø. et al. The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner Nature Commun. 9(1):3279 (2018)
- Luk, A., et al. CTC-mRNA (AR-V7) Analysis from Blood Samples—Impact of Blood Collection Tube and Storage Time. Int. J. Mol. Sci. 18(5), 1047 (2017)
- Lampignano R. et al. Diagnostic Leukapheresis Enables Reliable Transcriptomic Profiling of Single Circulating Tumor Cells to Characterize Inter-Cellular Heterogeneity in Terms of Endocrine Resistance [Parsortix and CellCelector]. Int. J. Mol. Sci. 18, 1885 (2017)
- Giannopoulou, L. et al. Liquid biopsy in ovarian cancer: recent advances on circulating tumor cells and circulating tumor DNA (Review) Clin. Chem. Lab. Med. Epub (2017)
- Ao Z. & Liu X. Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells. In: Circulating Tumor Cells, 235–246 (2017)
- Ma, Y. et al. Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies Int. J. Mol. Sci. 17(8): E1264 (2016)
- Neumann, M.H. et al. Isolation and characterization of circulation tumor cells using a novel workflow combining CellSearch® and CellCelectorTM Biotechnol Prog. 33(1): 125-132 (2016)
- Blassl, C. et al. Gene expression profiling of single circulating tumor cells in ovarian cancer - Establishment of a multi-marker gene panel Molecular Oncology 10(7): 1030-42 (2016)
- Schneck, H. et al. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer PLoS One 10(12): e0144535 (2015)
- Lohr, G.J. et al. Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer Nature Biotechnology 32: 479-484 (2014)
- Yao, X. et al. Tumor cells are dislodged into the pulmonary vein during lobectomy J. Thorac Cardiovasc. Surg. 148(6): 3224-31 (2014)
- Heidary, M. et al. The dynamic range of circulating tumor DNA in metastatic breast cancer Breast Cancer Res. 16(4): 421 (2014)
- Adalsteinsson, V.A. et al. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR + CXC chemokines Integr. Biol. (Camb.) 5(10): 1272-81 (2013)
- Lampignano, R. et al. The combination of ParsortixTM and CellCelectorTM enables the characterisation of EpCAMneg CTCs in breast cancer Poster ISMRC 2016 Hamburg
- Neumann, M. et al. CellCelector isolation of CTCs enables additional marker staining followed by panel sequencing Poster ISMRC 2016 Hamburg
- Neumann, M. et al. CellCelector and CellSearch combined workflow Poster ACTC 2014 Crete
- Voigt, K. et al. Single cell sequencing of rare cells after magnetic enrichment Poster ISRMC 2013 Paris
- Berger, T. et al. Detection and characterization of CTCs in patients with hepatocellular carcinoma (HCC) Poster DGHO 2013 Vienna
Isolierung seltener Einzelzellen: fetale Zellen (cb NIPT)
- Toft, C.L.F. et al. Cell-based non-invasive prenatal testing for monogenic disorders: confirmation of unaffected fetuses following preimplantation genetic testing J Assist Reprod Genet 38, 1959–1970 (2021)
- Jeppesen L.D., et al. Cell-based non-invasive prenatal diagnosis in a pregnancy at risk of cystic fibrosis Prenat Diagn. 2021 Jan;41(2):234-240
- Hatt L, et al. Cell-based noninvasive prenatal testing (cbNIPT) detects pathogenic copy number variations Clin Case Rep. 2020
- Singh R. et. al. Laboratory challenges with cell-based NIPT ISPD Global Updates, November 2018
- Vestergaard E.M. et al. On the road to replacing invasive testing with cell‐based NIPT: Five clinical cases with aneuploidies, microduplication, unbalanced structural rearrangement, or mosaicism Prenatal Diagnostics 37(11):1120-1124 (2017)
- Kølvraa, S. et al. Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women Prenatal Diagnostics 36: 1-8 (2016)
Zellabstammung und Einzelzellisolierung nach Zellteilung
- Kull T., et al. NfκB signaling dynamics and their target genes differ between mouse blood cell types and induce distinct cell behavior. Blood, 140(2):99-111, (2022)
- Wehling A. et al. Combined single-cell tracking and omics improves blood stem cell fate regulator identification. Blood, Pre-print (2022)
- Tao L., Raz O. et al. A biological-computational human cell lineage discovery platform based on duplex molecular inversion probes. Biorxiv, Pre-print (2018)
Einzelzellisolierung verschiedenes
- Chenxin Li et al. Single-cell multi-omics enabled discovery of alkaloid biosynthetic pathway genes in the medical plant Catharanthus roseus bioRxiv, 2022
- Takashima, S., et al. Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells Sci Rep 11, 1520 (2021)
- Tao, L., Retrospective cell lineage reconstruction in humans by using short tandem repeats Cell Reports Methods 1, 100054, (2021)
- Baron, C.S. et al. Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in aorta Nature Commun. 9(1): 2517 (2018)
- Biezuner, T., et al. Comparison of seven single cell Whole Genome Amplification commercial kits using targeted sequencing. Biorxiv (2017)
- Wang, J. et al. Single cell sequencing: a distinct new field [Review] Clin. Trans. Med. 6(1): 10 Epub Feb 20 (2017)
- Ku, C.J. et al. GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion Mol Cell Biol. Epub Mar 20 (2017)
- Ellsworth, D.L. et al. Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis (Review). Clinical and Translational Medicine 6, 15 (2017)
- Dura, B. et al. Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture PNAS 113(26): E3599-608 (2016)
- Liao, M.C. et al. Single-Cell Detection of Secreted Aß and sAPPa from Human IPSC-Derived Neurons and Astrocytes J. Neurosci. 36(5): 1730-46 (2016)
- Wang, Y. et al. Advances and applications of single-cell sequencing technologies (Review) Molecular Cell. 58(4): 598-609 (2015)
- Castellarnau, M. et al. Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis Small 11(4): 489-498 (2015)
- Navin, N.E. Cancer genomics: one cell at a time (Review) Genome Biol. 15(8): 452 (2014)
- Cornils, K. et al. Multiplexing clonality - combining RGB marking and genetic barcoding Nucl. Acid Res. 42(7): e56 (2014)
- Wuethrich, I. et al. A Mouse Monoclonal Antibody Against Alexa Fluor 647 Monoclonal Antibodies in Immunodiagnosis and Immuntherapy 33(2): 109-120 (2014)
- Kluth, S.M. et al. Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1 Stem Cells Int. 985285 (2013)
- Zoldan, K. et al. Visualizing of migration, interaction, proliferation or differentation of cells in time lapse exposures using the cell separation robot CellCelectorTM nature methods application notes 2010
- Wagner, K. et al. Selection of reference genes for quantitative PCR of single cells Poster STS 2011
Hochdurchsatz-Wirkstoffscreening
- Avital-Shmilovici M. et al. A Mega-High-Throughput Screening Platform for the Discovery of Biologically Relevant Sequence-Defined Non-Natural Polymers ChemRxiv. (2021) Preprint
Low-volume Isolierung von Kolonien aus Flüssigkulturen
- Machado H.E. et al. Genome-wide mutational signatures of immunological diversification in normal lymphocytes bioRxiv (2021) Preprint
Imaging
- Schenk, M.W. et al. Soluble guanylate cyclase signalling mediates etoposide resistance in progressing small cell lung cancer Nat Commun. 12, 6652 (2021)
- Lemmens I. et al The Development of RNA-KISS, a Mammalian Three-Hybrid Method to Detect RNA–Protein Interactions in Living Mammalian Cells J. Proteome Res., 19, 7, 2529–2538 (2020)
- Sand, N.T. et al. Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells. Int. J. Mol. Sci. 21(7), 2401 (2020)
- Nett, I. et al. Negative feedback via RSK modulates Erk-dependent progression from naïve pluripotency. EMBO Reports 19:e45642 (2018)