Antibody discovery / Nanowell-based B-cell and T-cell screening and isolation

  • Nelep C. Fast Antibody Discovery Workflows on the CellCelector™ Flex Platform Poster PEGS Europe (2021)

Cell line development incl. Nanowell-based single cell cloning

  • Broussau, S., Nelep, C. & Gilbert, R. Improved Packaging Cell Line Development for Lentivirus Production by CellCelector™ Nanowell-based Single Cell Cloning Technology. Poster Festival of Biologics (2019)

  • Winkler, K. et al. Evaluation of the HT-NIC Technology to Generate Monoclonal Producer Cell Lines. Poster ESACT (2019)

Adherent cell colony isolation (stem cells, tumor cells, HEK293, etc.)

  • Zoldan, K. et al. Automated harvest of induced pluripotent stem cell colonies and colony fractions using the cell separation robot CellCelectorTM  nature methods application notes (2010)  

  • Zoldan, K. et al. Automated isolation of semi-adherent  macrophage-like cells from a fibroblast-contaminated culture using the cell separation robot CellcelectorTM nature methods application notes (2010)  

  • Haupt, S. et al. Automated selection and harvesting of pluripotent stem cell colonies using the CellCelector  Nature Methods (2009)   

  • Planes, E. et al. Life Cell Imaging for Quality Control of Pluripotent Stem Cell Culture Poster ISSCR 2013 Boston

Clone picking from semi-solid media (CHO, hybridoma, etc.)

  • Zoldan, K. et al. Automated clonal selection of  high-producing hybridoma colonies from methylcellulose-based, semi-solid  medium using the cell separation robot CellCelectorTM  nature methods application notes (2010)   

  • Julien, A. et al. Antigen specific antibody-producing clones selected by isotypes  Biosystems International Application Note  

  • Gilbert, R. Fluorescent labeling in Semi-Solid Medium to Identify High Expressing Clones for Recombinant Protein Production (CHO)  CHI's Bioprocessing Summit Presentation, Boston 2011

Spheroid isolation and manipulation

Single cell CRISPR gene editing - single cell cloning

Rare single cell isolation: circulating tumor cells (CTCs)

  • Lampignano, R. et al. The combination of ParsortixTM and CellCelectorTM enables the characterisation of EpCAMneg CTCs in breast cancer  Poster ISMRC 2016 Hamburg

  • Neumann, M. et al. CellCelector isolation of CTCs enables additional marker staining followed by panel sequencing Poster ISMRC 2016 Hamburg

  • Neumann, M. et al. CellCelector and CellSearch combined workflow  Poster ACTC 2014 Crete

  • Voigt, K. et al. Single cell sequencing of rare cells after magnetic enrichment  Poster ISRMC 2013 Paris

  • Berger, T. et al. Detection and characterization of CTCs in patients with hepatocellular carcinoma (HCC)  Poster DGHO 2013 Vienna

Rare single cell isolation: fetal cells (cb NIPT)

Cell lineage and single cell isolation after cell division

Single cell isolation misc.

  • Zoldan, K. et al. Visualizing of migration, interaction,  proliferation or differentation of cells in time lapse exposures using  the cell  separation robot CellCelectorTM nature methods application notes 2010  

  • Wagner, K. et al. Selection of reference genes for quantitative PCR of single cells  Poster STS 2011

High-throughput compound screening

Low-volume liquid culture colony harvesting